Search results for "normal human"

showing 6 items of 6 documents

Indicaxanthin from Opuntia Ficus Indica (L. Mill) impairs melanoma cell proliferation, invasiveness, and tumor progression.

2018

Abstract Background: A strong, reciprocal crosstalk between inflammation and melanoma has rigorously been demonstrated in recent years, showing how crucial is a pro-inflammatory microenvironment to drive therapy resistance and metastasis. Purpose: We investigated on the effects of Indicaxanthin, a novel, anti-inflammatory and bioavailable phytochemical from Opuntia Ficus Indica fruits, against human melanoma both in vitro and in vivo. Study Design and Methods: The effects of indicaxanthin were evaluated against the proliferation of A375 human melanoma cell line and in a mice model of cutaneous melanoma. Cell proliferation was assessed by MTT assay, apoptosis by Annexin V-Fluorescein Isothio…

0301 basic medicine3003MaleSkin NeoplasmsPyridinesPyridinePhytochemicalsMelanoma ExperimentalPharmaceutical ScienceIndicaxanthinApoptosisBcl-2 B cell lymphoma gene-2 (Bcl-2)chemistry.chemical_compoundMice0302 clinical medicineOpuntia Ficus Indica (L.Mill)Settore BIO/10 - BiochimicaDrug DiscoveryCXCL1 chemokine (C-X-C motif) ligand 1MelanomaNF-κB nuclear factor kappa BMTT 3-[45-dimethyltiazol-2-yl]-25-diphenyl tetrazolium bromideMelanomaNF-kappa BOpuntiaComplementary and Alternative Medicine2708 DermatologyBetaxanthinsCXCL1030220 oncology & carcinogenesisMolecular MedicinePhC phytochemicalGrowth inhibitionIndicaxanthinHumanBiologyPhytochemicalNHEM normal human epidermal melanocyte03 medical and health sciencesc-FLIP FLICE-inhibitory proteinIn vivoCell Line TumormedicineAnimalsHumansNeoplasm InvasivenessSkin NeoplasmCell ProliferationNeoplasm InvasiveneInflammationPharmacologyCell growthAnimalDrug Discovery3003 Pharmaceutical ScienceApoptosimedicine.diseaseMice Inbred C57BL030104 developmental biologyComplementary and alternative medicinechemistryTumor progressionList of Abbrevations: AxV-FITC annexin V-fluorescein isothiocyanateBetaxanthinFruitCutaneous melanomaCancer researchPI propidium iodide PIPhytomedicine : international journal of phytotherapy and phytopharmacology
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Identification of ELF3 as an early transcriptional regulator of human urothelium

2014

AbstractDespite major advances in high-throughput and computational modelling techniques, understanding of the mechanisms regulating tissue specification and differentiation in higher eukaryotes, particularly man, remains limited. Microarray technology has been explored exhaustively in recent years and several standard approaches have been established to analyse the resultant datasets on a genome-wide scale. Gene expression time series offer a valuable opportunity to define temporal hierarchies and gain insight into the regulatory relationships of biological processes. However, unless datasets are exactly synchronous, time points cannot be compared directly.Here we present a data-driven ana…

Hepatocyte Nuclear Factor 3-alphaTime seriesTime FactorsPPARγMicroarrayNormal Human UrotheliumComputational biologyBiologyReal-Time Polymerase Chain ReactionBioinformaticsProto-Oncogene ProteinsGene expressionElectric ImpedanceTranscriptional regulationHumansRNA Small InterferingGeneTranscription factorMolecular BiologyDNA PrimersGene knockdownProto-Oncogene Proteins c-etsReverse Transcriptase Polymerase Chain ReactionMicroarray analysis techniquesGene Expression Regulation DevelopmentalCell DifferentiationCell BiologyMicroarray AnalysisImmunohistochemistryELF3DNA-Binding ProteinsDifferentiationGene Knockdown TechniquesGene chip analysisGene expressionUrotheliumTranscription FactorsDevelopmental BiologyDevelopmental Biology
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Head-to-head comparison of plasma cTnI concentration values measured with three high-sensitivity methods in a large Italian population of healthy vol…

2019

Abstract Background The study aim is to compare cTnI values measured with three high-sensitivity (hs) methods in apparently healthy volunteers and patients admitted to emergency department (ED) with acute coronary syndrome enrolled in a large multicentre study. Methods Heparinized plasma samples were collected from 1511 apparently healthy subjects from 8 Italian clinical institutions (mean age: 51.5 years, SD: 14.1 years, range: 18–65 years, F/M ratio:0.95). All volunteers denied chronic or acute diseases and had normal values of routine laboratory tests. Moreover, 1322 heparinized plasma sample were also collected by 9 Italian clinical institutions from patients admitted to ED with clinica…

Male0301 basic medicineprincipal component analysisvery elderlyClinical BiochemistryheparinizationBiochemistryPatient Admission0302 clinical medicineReference ValuesLimit of Detectionblood analysisTroponin IHealthy volunteers80 and overMyocardial infarctionAcute coronary syndrome; Cardiac troponins; High-sensitivity methods; Myocardial infarction; Reference population values; Acute Coronary Syndrome; Adolescent; Adult; Aged; Aged 80 and over; Blood Chemical Analysis; Female; Humans; Italy; Male; Middle Aged; Myocardium; Patient Admission; Reference Values; Troponin I; Young Adult; Emergency Service Hospital; Healthy Volunteers; Limit of DetectionReference population valuescomparative studyHigh-sensitivity methodsAged 80 and overemergency wardEmergency Servicehospital emergency servicetroponin I acute coronary syndromeSettore BIO/12clinical trialGeneral Medicinecardiac troponin 1 CLIAMiddle AgedHealthy Volunteerspriority journalItaly030220 oncology & carcinogenesisCardiac troponinFemaleAcute coronary syndromeEmergency Service HospitalAdultmedicine.medical_specialtyAcute coronary syndromecardiac muscleAdolescentHead to headheart infarctionArticleYoung AdultHospital03 medical and health sciencesbloodInternal medicinemedicinesexHumanscontrolled studyADVIA Centaurdiagnostic test accuracy studyhumannormal humanproceduresAgedbusiness.industryMyocardiumTroponin IBiochemistry (medical)reference valueEmergency departmentmedicine.diseasemajor clinical studyhospital admissionyoung adult Acute Coronary SyndromeHigh-sensitivity methodMyocardial infarctionmulticenter study030104 developmental biologySettore BIO/12 - Biochimica Clinica E Biologia Molecolare Clinicaageprotein blood levelReference valuesCardiac troponinsbusinessmetabolismBlood Chemical Analysis
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Plantar flexor activation capacity and H reflex in older adults: adaptations to strength training.

2002

The purpose of this study was to investigate whether the voluntary neural drive and the excitability of the reflex arc could be modulated by training, even in old age. To this aim, the effects of a 16-wk strengthening program on plantar flexor voluntary activation (VA) and on the maximum Hoffman reflex (Hmax)-to-maximum M wave (Mmax) ratio were investigated in 14 elderly men (65–80 yr). After training, isometric maximum voluntary contraction (MVC) increased by 18% ( P < 0.05) and weight-lifting ability by 24% ( P < 0.001). Twitch contraction time decreased by 8% ( P < 0.01), but no changes in half relaxation time and in peak twitch torque were observed. The VA, assessed by twitch …

MaleVolitionAgingPhysiologydegenerationadaptationIsometric exerciseNerve conduction velocityhuman experimentH-ReflexTriceps surae muscleTwitch interpolationadaptation; aged; aging; article; controlled study; degeneration; excitability; exercise; Hoffmann reflex; human; human experiment; male; muscle contraction; muscle isometric contraction; muscle relaxation; muscle twitch; nerve conduction; normal human; plantaris muscle; priority journal; reflex arc; strength; torque; training; voluntary movement; Aging; Exercise; Triceps surae; Twitch interpolationAged 80 and overtrainingvoluntary movementReflex arcarticlereflex arcmusculoskeletal systemAdaptation Physiologicalmuscle twitchmedicine.anatomical_structuremuscle relaxationpriority journalCardiologyTriceps suraemedicine.symptomstrengthMuscle contractionMuscle Contractionmedicine.medical_specialtyWeight Liftingplantaris muscleStrength trainingPhysical exercisePhysical medicine and rehabilitationPhysiology (medical)Internal medicineexcitabilitymedicineHumanscontrolled studyhumannormal humanMuscle SkeletalExercisenerve conductionAgedmuscle isometric contractionbusiness.industrybody regionsHoffmann reflexTorqueH-reflexbusinessJournal of applied physiology (Bethesda, Md. : 1985)
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Patterns of male-specific inter-population divergence in Europe, West Asia and North Africa

2000

summary We typed 1801 males from 55 locations for the Y-specific binary markers YAP, DYZ3, SRY "!)$" and the (CA)n microsatellites YCAII and DYS413. Phylogenetic relationships of chromosomes with the same binary haplotype were condensed in seven large one-step networks, which accounted for 95% of all chromosomes. Their coalescence ages were estimated based on microsatellite diversity. The three largest and oldest networks undergo sharp frequency changes in three areas. The more recent network 3‐1A clearly discriminates between Western and Eastern European populations. Pairwise Fst showed an overall increment with increasing geographic distance but with a slope greatly reduced when compared …

MalehaplotypePopulation geneticsVariation (Genetics)phylogenyAfrica NorthernModelsY Chromosomegenetic variabilitypopulation dynamicsNorthernDinucleotide RepeatsGenetics (clinical)education.field_of_studyPhylogenetic treeGeographyarticlechromosome analysislinguisticsStatisticalEastern europeanEuropeGeographypriority journalMicrosatelliteWesternmarker geneAsiaEvolutionPopulationPopulationmicrosatellite DNA; article; Asia; chromosome analysis; controlled study; Europe; genetic variability; geographic distribution; haplotype; human; linguistics; male; marker gene; normal human; North Africa; phylogeny; population dynamics; priority journal; Africa Northern; Asia Western; Dinucleotide Repeats; Europe; Evolution Molecular; Genetics Population; Geography; Haplotypes; Humans; Male; Microsatellite Repeats; Models Genetic; Models Statistical; Variation (Genetics); Y ChromosomeY chromosomeEvolution MolecularGeneticGeographical distancegeographic distributionAsia WesternGeneticsHumanscontrolled studyhumannormal humaneducationModels StatisticalModels GeneticHaplotypeGenetic VariationMolecularNorth AfricaSettore BIO/18 - GeneticaGenetics PopulationHaplotypesEvolutionary biologyAfricamicrosatellite DNAMicrosatellite Repeats
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Overestimation of numerical distances in the left space

2004

Normal subjects presented with a middle number and two left- and right-sided outer numbers overestimate the numerical distance between the middle number and that positioned at its left side. Repetitive transcranial magnetic stimulation (rTMS) of the right posterior parietal cortex specifically counteracts this bias, suggesting that the mental representation of space defined by numbers is shifted toward the left side depending on a greater activity of the right hemisphere.

Settore M-PSI/02 - Psicobiologia E Psicologia Fisiologicabiparietal distance female human human experiment left hemisphere male normal human parietal lobe priority journal right hemisphere transcranial magnetic stimulation
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